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Clinical and Diagnostic Laboratory Immunology, January 2000, p. 91-95, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Cloning and Nucleotide Sequence Analysis of a Novel Human Papillomavirus (Type 82) Associated with Vaginal Intraepithelial Neoplasia

Nao Kino,1 Tetsutaro Sata,1 Yuko Sato,1 Motoyasu Sugase,2 and Toshihiko Matsukura3,*

Department of Pathology and Laboratory of Pathology, AIDS Research Center,1 and Laboratory of Tumor Viruses, Department of Virology II,3 National Institute of Infectious Diseases, Tokyo, and Department of Obstetrics and Gynecology, Nagano Red Cross Hospital, Nagano,2 Japan

Received 26 July 1999/Returned for modification 6 October 1999/Accepted 4 November 1999

The genome of a novel human papillomavirus (HPV-82) was cloned from a vaginal intraepithelial neoplasia grade I. In our series of 291 biopsy specimens, HPV-82 was identified in one case each of cervical intraepithelial neoplasia grade II and grade III by blot hybridization. The histological localization of HPV-82 DNA in the three lesions was confirmed by in situ hybridization. The results indicated that HPV-82 is an etiologic agent for vaginal and cervical intraepithelial neoplasia. By nucleotide sequence similarity of L1 open reading frame (ORF), HPV-82 was closely related to HPV-26, -51, and -69. To know the precise relationship between the HPVs, we determined the complete sequence of HPV-82, as well as that of HPV-69. Sequencing revealed that the four HPVs had no initiation codon in the E5 ORF and had extensive nucleotide sequence similarities in all ORFs. In addition, they exhibited unique frame position patterns for ORFs, different from those of the other genital HPVs.

* Corresponding author. Mailing address: Laboratory of Tumor Viruses, Department of Virology II, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. Phone: 81 3 5285 1111. Fax: 81 3 5285 1161. E-mail: toshi{at}nih.go.jp.

Clinical and Diagnostic Laboratory Immunology, January 2000, p. 91-95, Vol. 7, No. 1
1071-412X/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Menzo, S., Monachetti, A., Trozzi, C., Ciavattini, A., Carloni, G., Varaldo, P. E., Clementi, M. (2001). Identification of Six Putative Novel Human Papillomaviruses (HPV) and Characterization of Candidate HPV Type 87. J. Virol. 75: 11913-11919 [Abstract] [Full Text]  


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