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Clinical and Diagnostic Laboratory Immunology, March 2000, p. 218-225, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cytoskeletal Alterations in Lipopolysaccharide-Induced Bovine Vascular Endothelial Cell Injury and Its Prevention by Sodium Arsenite

Dipshikha Chakravortty, Naoki Koide, Yutaka Kato, Tsuyoshi Sugiyama, Makoto Kawai, Masako Fukada, Tomoaki Yoshida, and Takashi Yokochi*

Department of Microbiology and Immunology and Division of Bacterial Toxin, Research Center for Infectious Disease, Aichi Medical University, Nagakute, Aichi 480-1195, Japan

Received 7 September 1999/Accepted 30 December 1999

Morphological changes, especially cytoskeletal alterations, in lipopolysaccharide (LPS)-induced vascular endothelial cell injury were studied by using LPS-susceptible bovine aortic endothelial cells (BAEC). BAEC in cultures with LPS showed cell rounding, shrinking, and intercellular gap formation. In those cells, LPS caused the disorganization of actin, tubulin, and vimentin. LPS also induced a reduction in the F-actin pool and an elevation in the G-actin pool. Cytoskeletal disorganization affected transendothelial permeability across the endothelial monolayer. Pretreatment of BAEC with sodium arsenite (SA) prevented alterations in LPS-induced BAEC injury. However, posttreatment with SA had no protective effect on them. SA upregulated the expression of heat shock protein in the presence of LPS. The role of SA in prevention of LPS-induced BAEC injury is discussed.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Aichi Medical University, Aichi 480-1195, Japan. Phone: 81 (561) 62-3311. Fax: 81 (561) 63-9187. E-mail: yokochi{at}amugw.aichi-med-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, March 2000, p. 218-225, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chakravortty, D., Kato, Y., Sugiyama, T., Koide, N., Mu, M. M., Yoshida, T., Yokochi, T. (2001). The Inhibitory Action of Sodium Arsenite on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 267.4 Macrophage Cells: A Role of Raf-1 in Lipopolysaccharide Signaling. J. Immunol. 166: 2011-2017 [Abstract] [Full Text]  


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