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Clinical and Diagnostic Laboratory Immunology, March 2000, p. 307-311, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Measurement of T-Lymphocyte Responses in Whole-Blood Cultures Using Newly Synthesized DNA and ATP

P. R. Sottong,1,* J. A. Rosebrock,2 J. A. Britz,1 and T. R. Kramer3

Cylex Inc., Columbia, Maryland 210451; ImmTech, Inc., New Windsor, Maryland 217622; and U.S. Department of Agriculture, Little Rock, Arkansas 722113

Received 19 July 1999/Returned for modification 18 October 1999/Accepted 14 December 1999

The proliferative response is most frequently determined by estimating the amount of [3H]thymidine incorporated into newly synthesized DNA. The [3H]thymidine procedure requires the use of radioisotopes as well as lengthy periods of incubation (>72 h). An alternative method of assessing T-lymphocyte activation in whole-blood cultures involves the measurement of the nucleotide ATP instead of [3H]thymidine incorporation. In addition, the Luminetics assay of T-cell activation measures specific T-lymphocyte subset responses through the use of paramagnetic particles coated with monoclonal antibodies against CD antigens. This assay permits rapid (24 h) analysis of lymphocyte subset activation responses to mitogens and recall antigens in small amounts of blood.

* Corresponding author. Mailing address: Cylex Inc., 8970 Old Annapolis Rd., Columbia, MD 21045. Phone: (410) 964-0236. Fax: (410) 964-0367. E-mail: contact{at}cyclex.net.

Clinical and Diagnostic Laboratory Immunology, March 2000, p. 307-311, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Barna, B. P., Culver, D. A., Yen-Lieberman, B., Dweik, R. A., Thomassen, M. J. (2003). Clinical Application of Beryllium Lymphocyte Proliferation Testing. CVI 10: 990-994 [Full Text]  


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