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Clinical and Diagnostic Laboratory Immunology, March 2000, p. 322-324, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Description of a Nonlethal Herpes Simplex Virus Type 1 Glycoprotein D Deletion Mutant Affecting a Site Frequently Used for PCR

P. V. Coyle,* S. Jain, D. Wyatt, C. McCaughey, and H. J. O'Neill

Regional Virus Laboratory, Royal Victoria Hospital, Belfast, United Kingdom

Received 17 September 1999/Returned for modification 11 November 1999/Accepted 29 December 1999

We report a herpes simplex virus type 1 mutant which failed to amplify with a commonly used glycoprotein D primer set. The virus contained a nine-base deletion in the gene's 5' nontranslated region. The altered amplicon was clearly distinguishable on a 4% high-resolution agarose gel.

* Corresponding author. Mailing address: Regional Virus Laboratory, Royal Victoria Hospital, Grosvenor Road, Belfast BT12 6BN, United Kingdom. Phone: 44 1232 894628. Fax: 44 1232 439181. E-mail: p.coyle{at}qub.ac.uk.

Clinical and Diagnostic Laboratory Immunology, March 2000, p. 322-324, Vol. 7, No. 2
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Li, Y., Pan, Z., Ji, Y., Sheppard, M., Jeffries, D. J., Archard, L. C., Zhang, H. (2003). Herpes Simplex Virus Type 1 Infection Associated with Atrial Myxoma. Am. J. Pathol. 163: 2407-2412 [Abstract] [Full Text]  
  • Madeley, C R, McCaughey, C., O'Neill, H. J, Wyatt, D. E, Coyle, P. V, Curry, A., Caul, E O., Morgan-Capner, P. (2000). Rationalised virological electron microscope specimen testing policy. J. Clin. Pathol. 53: 722-723 [Full Text]  


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