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Clinical and Diagnostic Laboratory Immunology, May 2000, p. 396-403, Vol. 7, No. 3
Medcenter One Health Systems, Bismarck, North
Dakota 58501,1 and Department of
Microbiology and Immunology, University of North Dakota School of
Medicine and Health Sciences, Grand Forks, North Dakota
582022
Received 19 October 1999/Returned for modification 30 December
1999/Accepted 26 January 2000
An indirect fluorescence antibody (IFA) procedure was used for the
rapid detection of respiratory viruses in direct clinical specimens and
for determining the epidemiology of viruses in a community hospital
setting. Viral respiratory diseases were monitored for 10 consecutive
respiratory seasons. The Bartels Viral Respiratory Screening and
Identification Kit is an IFA method that contains pooled and individual
monoclonal antibodies for seven common respiratory viruses. Compared
with 8,670 conventional tube cell cultures, IFA staining of direct
patient specimens had an overall sensitivity of 84.2% and a
specificity of 87.7%. Yearly epidemics of respiratory syncytial virus
were seen with alternating short and long intervals between successive
periods when virus was isolated. Epidemics following short intervals
were more severe. Influenza A virus epidemics occurred yearly, and
influenza B virus activity was seen generally every other year. When
influenza A and influenza B viruses were cocirculating in a given
season, the months of peak activity of one virus were always within 1 month of the peak activity of the other virus. Parainfluenza virus type
1 was detected in the autumn of odd-numbered years, and parainfluenza
type 2 virus was seen usually in the autumn of even-numbered years.
Parainfluenza type 3 virus and adenovirus were the most ubiquitous
agents, with peak incidence occurring in the late winter to spring.
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Use of Monoclonal Antibodies for Rapid Diagnosis of
Respiratory Viruses in a Community Hospital
*
Corresponding author. Mailing address: Medcenter One
Health Systems, 222 N. 7th St., Bismarck, ND 58501. Phone: (701)
323-5847. Fax: (701) 323-5831. E-mail: kpirmen{at}home.com.
Present address: 10298 Grand Lake Rd. Cold Spring, MN 56320-8703.
Clinical and Diagnostic Laboratory Immunology, May 2000, p. 396-403, Vol. 7, No. 3
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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