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Clinical and Diagnostic Laboratory Immunology, July 2000, p. 698-699, Vol. 7, No. 4
1071-412X/00/$04.00+0

Diagnosis of Human Immunodeficiency Virus Type 1 Infection with Different Subtypes Using Rapid Tests

Susan Phillips,1 Timothy C. Granade,1 Chou-Pong Pau,1 Debra Candal,1 Dale J. Hu,2 and Bharat S. Parekh1,*

HIV Immunology and Diagnostics Branch1 and International Activity Branch,2 Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Received 6 January 2000/Returned for modification 22 March 2000/Accepted 3 April 2000

We evaluated six rapid tests for their sensitivity and specificity in diagnosing human immunodeficiency virus type 1 (HIV-1) infection using 241 specimens (172 HIV-1 positive, 69 HIV-1 negative) representing different HIV-1 subtypes (A [n = 40], B [n = 47], C [n = 28], E [n = 42], and F [n = 7]). HIVCHEK, Multispot, RTD and SeroStrip were 100% sensitive and specific. Capillus failed to identify two of eight subtype C specimens (overall sensitivity of 98.85%), while the SUDS test (the only test approved by the Food and Drug Administration) gave false-positive results for 5 of 69 seronegative specimens (specificity of 93.24%). Our results suggest that although rapid tests perform well in general, it may be prudent to evaluate a rapid test for sensitivity and specificity in a local population prior to its widespread use.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, HIV Immunology and Diagnostics Branch, Mailstop D12, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-3647. Fax: (404) 639-2660. E-mail: bsp1{at}cdc.gov.

Clinical and Diagnostic Laboratory Immunology, July 2000, p. 698-699, Vol. 7, No. 4
1071-412X/00/$04.00+0


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