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Clinical and Diagnostic Laboratory Immunology, July 2000, p. 700-702, Vol. 7, No. 4
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development of a Recombinant Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay for Quantification of Antibodies against Porcine Reproductive and Respiratory Syndrome Virus†

Steven B. Witte,1,Dagger Cindy Chard-Bergstrom,1 Thomas A. Loughin,2 and Sanjay Kapil1,*

Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine,1 and Department of Statistics, College of Arts and Sciences,2 Kansas State University, Manhattan, Kansas 66506

Received 1 February 2000/Returned for modification 14 March 2000/Accepted 4 April 2000

A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.

* Corresponding author. Mailing address: Department of Diagnostic Medicine/Pathobiology, 1800 Denison Ave., College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506. Phone: (785) 532-4457. Fax: (785) 532-4829. E-mail: Kapil{at}vet.ksu.edu.

dagger Contribution no. 00-129 J from the Kansas Agricultural Experiment Station.

Dagger Present address: Pfizer Animal Health, Groton, CT 06340.

Clinical and Diagnostic Laboratory Immunology, July 2000, p. 700-702, Vol. 7, No. 4
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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