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Clinical and Diagnostic Laboratory Immunology, September 2000, p. 759-763, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Shipment Impairs Lymphocyte Proliferative Responses to Microbial Antigens

Rebecca A. Betensky,1 Elizabeth Connick,2 Jennifer Devers,3 Alan L. Landay,4 Mostafa Nokta,5 Susan Plaeger,6 Howard Rosenblatt,7 John L. Schmitz,8 Fred Valentine,9 Diane Wara,10 Adriana Weinberg,2 and Howard M. Lederman3,*

Harvard School of Public Health, Boston, Massachusetts1; University of Colorado Health Sciences Center, Denver, Colorado2; Johns Hopkins University School of Medicine, Baltimore, Maryland3; Rush Medical College, Chicago, Illinois4; University of Texas Medical Branch, Galveston,5 and Baylor College of Medicine, Houston,7 Texas; UCLA School of Medicine, Los Angeles,6 and UCSF School of Medicine, San Francisco,10 California; University of North Carolina, Chapel Hill, North Carolina8; and New York University Medical Center, New York, New York9

Received 21 December 1999/Returned for modification 18 February 2000/Accepted 25 May 2000

Lymphocyte proliferation assays (LPAs) are widely used to assess T-lymphocyte function of patients with human immunodeficiency virus infection and other primary and secondary immunodeficiency disorders. Since these assays require expertise not readily available at all clinical sites, specimens may be shipped to central labs for testing. We conducted a large multicenter study to evaluate the effects of shipping on assay performance and found significant loss of LPA activity. This may lead to erroneous results for individual subjects and introduce bias into multicenter trials.

* Corresponding author. Mailing address: Division of Pediatric Immunology, Johns Hopkins Hospital-CMSC 1102, 600 N. Wolfe St., Baltimore, MD 21287-3923. Phone: (410) 955-5883. Fax: (410) 955-0229. E-mail: Hlederma{at}jhmi.edu.

Clinical and Diagnostic Laboratory Immunology, September 2000, p. 759-763, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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