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Clinical and Diagnostic Laboratory Immunology, September 2000, p. 774-777, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Serotype-Cross-Reactive Immunoglobulin M Responses in Dengue Virus Infections Determined by Enzyme-Linked Immunosorbent Assay

Masaru Nawa,1,* Ken-Ichiro Yamada,2 Tomohiko Takasaki,2 Toshitaka Akatsuka,1 and Ichiro Kurane2

Department of Microbiology, Saitama Medical School, Saitama 350-0495,1 and Department of Virology 1, National Institute of Infectious Diseases, Shinjyuku, Tokyo 162-8640,2 Japan

Received 1 February 2000/Returned for modification 4 April 2000/Accepted 31 May 2000

We developed immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assays (ELISAs) with four monovalent dengue virus antigens. We attempted to determine whether IgM responses in dengue virus infections are serotype specific or serotype cross-reactive. Serum samples from 14 confirmed dengue cases were examined. In these 14 cases, which consisted of 12 Japanese and 2 non-Japanese patients, infecting dengue virus serotypes were defined by reverse transcription-PCR. Thirteen of the 14 cases were IgM positive in ELISA. IgM responses were serotype cross-reactive in these 13 cases but were highest against infecting dengue virus serotype in 9 of the 13 cases. These results indicate that IgM responses are generally dengue serotype cross-reactive but that IgM levels are highest against the infecting serotype in most dengue cases.

* Corresponding author. Mailing address: Department of Microbiology, Saitama Medical School, 38 Moroyama, Saitama 350-0495, Japan. Phone: 81 492 76 1166. Fax: 81 492 95 9107. E-mail: mnawa{at}saitama-med.ac.jp.

Clinical and Diagnostic Laboratory Immunology, September 2000, p. 774-777, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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