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Clinical and Diagnostic Laboratory Immunology, September 2000, p. 828-831, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of Primary Binding Assays for Presumptive Serodiagnosis of Swine Brucellosis in Argentina

P. Silva Paulo,1,* A. M. Vigliocco,1 R. F. Ramondino,1 D. Marticorena,1 E. Bissi,1 G. Briones,1 C. Gorchs,1 D. Gall,2 and K. Nielsen2

Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Ciudad de Buenos Aires, Argentina,1 and Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada K2H 8P92

Received 29 November 1999/Returned for modification 10 March 2000/Accepted 17 May 2000

An indirect enzyme-linked immunosorbent assay (IELISA), a competitive ELISA (CELISA), and a fluorescence polarization assay (FPA) for the presumptive serological diagnosis of swine brucellosis were evaluated using two populations of swine sera: sera from brucellosis-free Canadian herds and sera from Argentina selected based on positive reactions in the buffered antigen plate agglutination test (BPAT) and the 2-mercaptoethanol (2-ME) test. In addition, sera from adult swine from which Brucella suis was isolated at least once for each farm of origin were evaluated. The IELISA, CELISA, and FPA specificity values were 99.9, 99.5, and 98.3%, respectively, and the IELISA, CELISA, and FPA sensitivity values relative to the BPAT and the 2-ME test were 98.9, 96.6, and 93.8%, respectively. Actual sensitivity was assessed by using 37 sera from individual pigs from which B. suis was cultured, and the values obtained were as follows: BPAT, 86.5%; 2-ME test, 81.1%; IELISA, 86.5%; CELISA, 78.5%; and FPA, 80.0%.

* Corresponding author. Mailing address: Comision Nacional de Energia Atomica Centro Atomico Ezeiza, Av. del Libertador 8250, CP 1429, Ciudad de Buenos Aires, Argentina. Phone: 54-11-4379-8531. Fax: 54-11-4379-8540. E-mail: silvapau{at}cae.cnea.gov.ar.

Clinical and Diagnostic Laboratory Immunology, September 2000, p. 828-831, Vol. 7, No. 5
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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