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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 904-908, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Novel Approach for Specific Detection of Herpes Simplex Virus Type 1 and 2 Antibodies and Immunoglobulin G and M Antibodies

Bella Ohana,1,* Mark Lipson,1 Noga Vered,1 Isaac Srugo,2 Moshe Ahdut,3 and Abraham Morag3,dagger

Savyon Diagnostics Ltd., Ashdod 77610,1 Clinical Microbiology Laboratory, Bnai Zion Medical Center, Haifa 31048,2 and Clinical Virology Unit, Hadassah Medical Center, Jerusalem,3 Israel

Received 17 March 2000/Returned for modification 13 June 2000/Accepted 15 August 2000

Recently a few new herpes simplex virus (HSV) type-specific serological diagnostic tests have been introduced to the commercial market, but these tests have some limitations. Moreover, it is not yet clear which commercial test can be regarded as a "gold standard" for the serodiagnosis of HSV infections. In order to improve the clinical diagnostic value of serological tests for the detection of HSV infections, we developed novel, competition-based enzyme-linked immunosorbent assays for the specific determination of HSV type 2 antibodies (SeroHSV2) and HSV type 1 antibodies (SeroHSV1) and two complementary tests for the detection of HSV immunoglobulin M (IgM) and IgG antibodies (SeroHSV IgM and SeroHSV IgG). These four new kits were evaluated in comparison with some commercial kits for the detection of HSV antibodies that are commonly used at present in Israeli clinical laboratories. The results indicate that SeroHSV2 is highly sensitive (>92%) and highly specific (>94%). SeroHSV2 does not cross-react with other alphaherpesvirus antibodies. SeroHSV1 is highly sensitive (>94%) and specific (>91%) compared to four commercial available kits. SeroHSV IgM is highly specific (>92%) in comparison with other commercial HSV IgM tests. The sensitivity of SeroHSV IgM ranges between 50 and 70% compared to these tests. Further investigation of the discrepant results obtained by using in-house competition tests indicated that SeroHSV IgM is more sensitive. SeroHSV IgG was also found to be highly sensitive (>94%) and highly specific (>92%) compared to the other commercial HSV IgG tests.

* Corresponding author. Mailing address: Savyon Diagnostics Ltd., Kiryat Minrav, 3 Habosem St., Ashdod 77610, Israel. Phone: 972-8-8562920. Fax: 972-8-8563258. E-mail: savyon_d{at}netvision.net.il.

dagger Deceased.

Clinical and Diagnostic Laboratory Immunology, November 2000, p. 904-908, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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