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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 940-944, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection and Quantification of Antibodies to Newcastle Disease Virus in Ostrich and Rhea Sera Using a Liquid Phase Blocking Enzyme-Linked Immunosorbent Assay

Ricardo Luiz Moro de Sousa,1,2 Helio José Montassier,3 and Aramis Augusto Pinto3,*

Graduate Program in Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo,1 and Fundação de Amparo à Pesquisa do Estado de São Paulo,2 São Paulo, and Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14870-000, Jaboticabal,3 São Paulo, Brazil

Received 22 May 2000/Returned for modification 19 July 2000/Accepted 24 August 2000

A liquid phase blocking ELISA (LPB-ELISA) was adapted for the detection and quantification of antibodies to Newcastle disease virus. Sera from vaccinated and unvaccinated commercial flocks of ostriches (Struthio camelus) and rheas (Rhea americana) were tested. The purified and nonpurified virus used as the antigen and the capture and detector antibodies were prepared and standardized for this purpose. The hemagglutination-inhibition (HI) test was regarded as the reference method. The cutoff point for the LPB-ELISA was determined by a two-graph receiver operating characteristic analysis. The LPB-ELISA titers regressed significantly (P < 0.0001) on the HI titers with a high correlation coefficient (r = 0.875). The two tests showed good agreement (kappa  = 0.82; P < 0.0001), relative sensitivity (90.91%) and specificity (91.18%), and accuracy (91.02%), suggesting that they are interchangeable.

* Corresponding author. Mailing address: Laboratório de Virologia e Imunologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane, Km 05, 14870-000, Jaboticabal, São Paulo, Brazil. Phone: 55-16-3232500, ext. 225. Fax: 55-16-3224275. E-mail: aramisap{at}asbyte.com.br.

Clinical and Diagnostic Laboratory Immunology, November 2000, p. 940-944, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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