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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 945-946, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antifreeze Solution Improves DNA Recovery by Preserving the Integrity of Pathogen-Infected Blood and Other Tissues

Diana Sara Leal-Klevezas,1 Irma Olivia Martínez-Vázquez,2 Baltazar Cuevas-Hernández,2 and Juan Pablo Martínez-Soriano3,*

Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco,1 Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León,2 and Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Irapuato, Guanajuato,3 Mexico

Received 31 May 2000/Returned for modification 23 August 2000/Accepted 7 September 2000

Preserving blood samples for shipping and later DNA extraction has been performed by cooling, freezing, drying, freeze-drying, and protease treatment, among other methods. Most methods to preserve field samples for further DNA extraction do not prevent cellular and DNA damage or are useful only in preserving them for short periods. This report introduces a novel method for blood and tissue that allows preservation in freezing temperatures for a prolonged period of time. The solution reported here (20% ethylene glycol-propylene glycol) preserves cells and tissues integrity, as judged by microscopic analysis, and improves DNA yield and quality.

* Corresponding author. Mailing address: Unidad de Biotecnología e Ingeniería Genética de Plantas del CINVESTAV-IPN, Apartado Postal 629, 36500 Irapuato, Guanajuato, Mexico. Phone: (52)4-623-9637. Fax: (52)4-624-5996. E-mail: jpms{at}ira.cinvestav.mx.

Clinical and Diagnostic Laboratory Immunology, November 2000, p. 945-946, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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