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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 977-979, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid Detection of Salmonella enterica Serovar Choleraesuis in Blood Cultures by a Dot Blot Enzyme-Linked Immunosorbent Assay

Kritsana Janyapoon,1 Sunee Korbsrisate,2,* Hatairat Thamapa,1 Sittichai Thongmin,1 Suwattana Kanjanahareutai,3 Niramol Wongpredee,4 and Suttipant Sarasombath2

Faculty of Medical Technology, Rangsit University,1 Departments of Immunology2 and Microbiology,4 Faculty of Medicine Siriraj Hospital, Mahidol University, and Microbiology Section, Department of Pathology, Rajavithi General Hospital,3 Bangkok, Thailand

Received 11 February 2000/Returned for modification 3 April 2000/Accepted 3 August 2000

A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella enterica serovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Choleraesuis were positive on testing by the ELISA. In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%.

* Corresponding author. Mailing address: Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Phone: 66-2-418-0569. Fax: 66-2-418-1636. E-mail: grsks{at}mahidol.ac.th.

Clinical and Diagnostic Laboratory Immunology, November 2000, p. 977-979, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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