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Clinical and Diagnostic Laboratory Immunology, November 2000, p. 983-986, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Monoclonal Antibody Binding to a Surface-Exposed Epitope on Cowdria ruminantium That Is Conserved among Eight Strains

Sankale Shompole,1,2,* Fred R. Rurangirwa,2 Anderson Wambugu,1 John Sitienei,1 Duncan M. Mwangi,3 Anthony J. Musoke,3 Suman Mahan,4 Clive W. Wells,3 and Travis C. McGuire2

Biotechnology and Immunology Laboratory, Kenya Agricultural Research Institute, Kabete1 and International Livestock Research Institute, Nairobi,3 Kenya; Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-70402; and University of Florida/USAID Heartwater Research Project, Harare, Zimbabwe4

Received 1 December 1999/Returned for modification 24 March 2000/Accepted 14 August 2000

Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodies (EB) were identified by enzyme-linked immunosorbent assay, and surface binding of one MAb (446.15) to intact EB was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. MAb 446.15 bound an antigen of approximately 43 kDa in immunoblots of eight geographically distinct strains. The MAb did not react with Ehrlichia canis antigens or uninfected bovine endothelial cell lysate and may be useful in diagnostic assays and vaccine development.

* Corresponding author. Present address: Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040. Phone: (509) 335-6320. Fax: (509) 335-8529. E-mail: shompole{at}vetmed.wsu.edu.

Clinical and Diagnostic Laboratory Immunology, November 2000, p. 983-986, Vol. 7, No. 6
1071-412X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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