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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 14-20, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.14-20.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Naturally Acquired Antibody Responses to the C-Terminal Region of Merozoite Surface Protein 1 of Plasmodium vivax in Korea

Jae-Won Park,1 Seung-Hwan Moon,1 Joon-Sup Yeom,2,dagger Kook-Jin Lim,3 Mi-Jin Sohn,3 Woo-Chul Jung,4,Dagger Young-Jung Cho,4 Ki-Won Jeon,4 Woong Ju,5,§ Chang-Seok Ki,4 Myoung-Don Oh,6,7,* and Kangwon Choe6,7

Korean Armed Forces Central Medical Research Institute, Yusong-gu, Daejeon,1 5th Infantry Division of the ROK Army, Jeongok-eup, Yeoncheon-gun, Kyonggi-do,2 LG Biotech Research Institute II, LG Chemical Ltd. Research Park, Yusong-gu, Daejon,3 Korean Armed Forces Medical Command, Boondang-gu, Seongnam-si, Kyonggi-do,4 Yeoncheon Health & Medical Center, Jeongok-eup, Yeoncheon-gun, Kyonggi-do,5 and Department of Internal Medicine, Seoul National University College of Medicine,6 and Clinical Research Institute, Seoul National University Hospital,7 Chongno-gu, Seoul 110-744, Republic of Korea

Received 4 May 2000/Returned for modification 15 August 2000/Accepted 20 September 2000

We expressed a protein in Saccharomyces cerevisiae in order to evaluate the humoral immune responses to the C-terminal region of the merozoite surface protein 1 of Plasmodium vivax. This protein (Pv20018) had a molecular mass of 18 kDa and was reactive with the sera of individuals with patent vivax malaria on immunoblotting analysis. The levels of immunoglobulin M (IgM) and IgG antibodies against Pv20018 were measured in 421 patients with vivax malaria (patient group), 528 healthy individuals from areas of nonendemicity (control group 1), and 470 healthy individuals from areas of endemicity (control group 2), using the indirect enzyme-linked immunosorbent assay (ELISA) method. To study the longevity of the antibodies, 20 subjects from the patient group were also tested for the antibody levels once a month for 1 year. When the cutoff values for seropositivity were determined as the mean + 3 × standard deviation of the antibody levels in control group 1, both IgG and IgM antibody levels were negative in 98.5% (465 of 472) of control group 2. The IgG and IgM antibodies were positive in 88.1% (371 of 421) and 94.5% (398 of 421) of the patient group, respectively. The IgM antibody became negative 2 to 4 months after the onset of symptoms, whereas the IgG antibody usually remained positive for more than 5 months. In conclusion, indirect ELISA using Pv20018 expressed in S. cerevisiae may be a useful diagnostic method for vivax malaria.

* Corresponding author. Mailing address: Department of Internal Medicine, Seoul National University College of Medicine, 28, Yongon-dong, Chongno-gu, Seoul 110-744, Republic of Korea. Phone: (82)-2-760-2945. Fax: (82)-2-762-9662. E-mail: mdohmd{at}snu.ac.kr.

dagger Present address: Division of Infectious Diseases, Department of Internal Medicine, Yonsei University, College of Medicine, Seodaemoon-gu, Seoul 120-749, Republic of Korea.

Dagger Present address: Department of Internal Medicine, Catholic University Medical College, Seocho-gu, Seoul 137-040, Republic of Korea.

§ Present address: Department of Obstetrics and Gynecology, Seoul National University Hospital, Chongno-gu, Seoul 110-744, Republic of Korea.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 14-20, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.14-20.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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