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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 174-177, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.174-177.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Immunodiagnosis of Human Fascioliasis by an Enzyme-Linked Immunosorbent Assay (ELISA) and a Micro-ELISA

Silvana Carnevale,* Mónica I. Rodríguez, Graciela Santillán, Jorge H. Labbé, Marta G. Cabrera, Enrique J. Bellegarde, Jorge N. Velásquez, Jorge E. Trgovcic, and Eduardo A. Guarnera

Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, ANLIS "Dr. Carlos G. Malbrán," 1281 Buenos Aires, Argentina

Received 3 April 2000/Returned for modification 28 July 2000/Accepted 20 September 2000

Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the serodiagnosis of human fascioliasis.

* Corresponding author. Mailing address: Lituania 520, 1826 Remedios de Escalada, Argentina. Phone: 54 11 4242 0883. Fax: 54 11 4301 7437. E-mail: jorsil{at}overnet.com.ar.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 174-177, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.174-177.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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