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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 190-191, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.190-191.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Production of a Mouse Antiserum to Bacteroides fragilis Enterotoxin Using a Recombinant Enterotoxin Precursor

Silvia Sandini, Anna Scotto d'Abusco, Roberto La Valle, and Annalisa Pantosti*

Laboratory of Bacteriology and Medical Mycology, Instituto Superiore di Sanità, Rome, Italy

Received 28 June 2000/Returned for modification 28 August 2000/Accepted 10 October 2000

The precursor of the Bacteroides fragilis metalloprotease enterotoxin was cloned and expressed in Escherichia coli, which was not able to process the precursor into the biologically active enterotoxin. Mouse antiserum elicited to the recombinant precursor reacted with the purified enterotoxin and with a crude enterotoxin preparation from an enterotoxigenic strain. The antiserum neutralized the cytotoxic activity of the enterotoxin in HT-29 cells.

* Corresponding author. Mailing address: Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: (39) 064990-2331. Fax: (39) 064938-7112. E-mail:pantosti{at}iss.it.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 190-191, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.190-191.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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