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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 203-205, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.203-205.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Correlation between Enzyme-Linked Immunosorbent Assay and Immunofluorescence Assay with Lytic Antigens for Detection of Antibodies to Human Herpesvirus 8

Simone Topino,1 Laura Vincenzi,2 Ivano Mezzaroma,1 Emanuele Nicastri,1 Massimo Andreoni,3 and Maria C. Sirianni1,*

Department of Clinical Immunology, University of Rome La Sapienza,1 IRCCS L. Spallanzani2 and Department of Infectious Diseases, University of Tor Vergata,3 Rome, Italy

Received 27 July 2000/Returned for modification 27 September 2000/Accepted 25 October 2000

The purpose of this study was to evaluate, in Kaposi's sarcoma patients, the correlation between antibody titers to the lytic antigens of human herpesvirus 8, as assessed by immunofluorescence assay, and values obtained by an enzyme immunoassay. The methods showed a stringent correlation, r = 0.625 (P < 0.001).

* Corresponding author. Mailing address: Department of Clinical Immunology, University of Rome "La Sapienza," Viale dell' Università 37, 00185 Rome, Italy. Phone: 39 06 49972037. Fax: 39 06 4466209. E-mail: sirianni{at}uniromal.it.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 203-205, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.203-205.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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