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Clinical and Diagnostic Laboratory Immunology, January 2001, p. 74-78, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.74-78.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Apoptosis in T-Lymphocyte Subsets in Human Immunodeficiency Virus-Infected Children Measured Immediately Ex Vivo and following In Vitro Activation

Tim Niehues,1,2 Thomas W. McCloskey,1 Jennifer Ndagijimana,2 Gerd Horneff,2 Volker Wahn,2 and Savita Pahwa1,*

Department of Pediatrics, Division of Allergy and Immunology, North Shore University Hospital/New York University School of Medicine, Manhasset, New York1, and Children's Hospital, Heinrich Heine University, Düsseldorf, Germany2

Received 2 May 2000/Returned for modification 21 August 2000/Accepted 5 October 2000

Phosphatidylserine molecules are translocated to the outer plasma membrane of lymphocytes undergoing apoptosis and can be detected by the binding of fluorochrome-conjugated annexin V. Using the annexin V assay, we examined CD4 and CD8 T cells from human immunodeficiency virus (HIV)-infected children for apoptosis upon isolation or following in vitro culture. Immediate ex vivo analysis or overnight culture showed significantly higher levels of apoptosis in CD8 cells than in CD4 cells. Following culture with the activating stimulus phytohemagglutinin or anti-CD3 monoclonal antibody, we observed an increase in the percentage of apoptotic CD4 cells, whereas there was no change in the rate of CD8 cell death. These results demonstrate that in HIV-infected children, CD8 apoptosis may occur at a greater rate than CD4 apoptosis in vivo; greater CD4 depletion may be observed due to more efficient mechanisms for peripheral lymphocyte replacement in the CD8 compartment. Furthermore, our data suggest that CD8 lymphocytes may be maximally activated in vivo, a condition which may lead to the exhaustion of CD8-mediated immunity. These findings clarify the differences between the CD4 and CD8 apoptotic responses to HIV.

* Corresponding author. Mailing address: North Shore University Hospital, 350 Community Drive, Manhasset, NY 11030. Phone: (516) 562-4641. Fax: (516) 562-2866. E-mail: spahwa{at}nshs.edu.

Clinical and Diagnostic Laboratory Immunology, January 2001, p. 74-78, Vol. 8, No. 1
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.1.74-78.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Kharbanda, M., McCloskey, T. W., Pahwa, R., Sun, M., Pahwa, S. (2003). Alterations in T-Cell Receptor V{beta} Repertoire of CD4 and CD8 T Lymphocytes in Human Immunodeficiency Virus-Infected Children. CVI 10: 53-58 [Abstract] [Full Text]  


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