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Clinical and Diagnostic Laboratory Immunology, March 2001, p. 432-436, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.432-436.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Duration of Sample Storage Dramatically Alters Expression of the Human Immunodeficiency Virus Coreceptors CXCR4 and CCR5

Sharon Shalekoff and Caroline T. Tiemessen*

AIDS Virus Research Unit, National Institute for Virology, and Department of Virology, University of the Witwatersrand, Johannesburg, South Africa

Received 18 August 2000/Returned for modification 25 September 2000/Accepted 6 November 2000

Expression of the chemokine receptors CXCR4 and CCR5 was monitored using EDTA-anticoagulated whole blood held for different time periods prior to fluorescent-antibody staining. When left overnight CXCR4 expression on leukocytes was substantially increased, whereas CCR5 expression was reduced. The results were similar when heparin and acid-citrate-dextrose were used as anticoagulants.

* Corresponding author. Mailing address: National Institute for Virology, Private Bag X4, Sandringham 2131, South Africa. Phone: (01027-11) 321-4200/85. Fax: (01027-11) 882-0596. E-mail: caroline{at}niv.ac.za.

Clinical and Diagnostic Laboratory Immunology, March 2001, p. 432-436, Vol. 8, No. 2
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.2.432-436.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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