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Clinical and Diagnostic Laboratory Immunology, May 2001, p. 496-498, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.496-498.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of a Latex Agglutination Kit (Virogen Rotatest) for Detection of Bovine Rotavirus in Fecal Samples†

Yousif Al-Yousif, Joe Anderson, Cindy Chard-Bergstrom, Adrian Bustamante, Margaret Muenzenberger, Kimberly Austin, and Sanjay Kapil*

Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506

Received 30 November 2000/Returned for modification 2 January 2001/Accepted 18 January 2001

The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect the presence of group A rotavirus antigen. Of the 63 samples screened by VI-FA, 33 (58%) tested positive for rotavirus antigen. When the results from the LAT were compared to those from VI-FA, the "gold standard" for detection of bovine rotavirus in fecal samples, the sensitivity and specificity were found to be 87.8 and 73.3%, respectively. Latex agglutination compared with ELISA (the reference method) showed 100% sensitivity and 96.3% specificity, and when ELISA was compared with VI, the sensitivity was 84.8% and the specificity was 73.3%. Latex agglutination is easy to perform in a short time and does not require expensive equipment or skilled personnel, and the reagents have long shelf lives. These factors make the LAT suitable and highly efficient for use in a clinical laboratory as a rapid screening test for bovine rotavirus.

* Corresponding author. Mailing address: Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5606. Phone: (785) 532-4457. Fax: (785) 532-4829. E-mail: kapil{at}vet.ksu.edu.

dagger Contribution 01-16-J from the Kansas Agricultural Station, Manhattan.

Clinical and Diagnostic Laboratory Immunology, May 2001, p. 496-498, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.496-498.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Maes, R. K., Grooms, D. L., Wise, A. G., Han, C., Ciesicki, V., Hanson, L., Vickers, M. L., Kanitz, C., Holland, R. (2003). Evaluation of a Human Group A Rotavirus Assay for On-Site Detection of Bovine Rotavirus. J. Clin. Microbiol. 41: 290-294 [Abstract] [Full Text]  
  • Al-Yousif, Y., Anderson, J., Chard-Bergstrom, C., Kapil, S. (2002). Development, Evaluation, and Application of Lateral-Flow Immunoassay (Immunochromatography) for Detection of Rotavirus in Bovine Fecal Samples. CVI 9: 723-725 [Abstract] [Full Text]  


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