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Clinical and Diagnostic Laboratory Immunology, May 2001, p. 612-615, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.612-615.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Canine Parvovirus (CPV) Vaccination: Comparison of Neutralizing Antibody Responses in Pups after Inoculation with CPV2 or CPV2b Modified Live Virus Vaccine

Annamaria Pratelli,1,* Alessandra Cavalli,1 Vito Martella,1 Maria Tempesta,1 Nicola Decaro,1 Leland Eugene Carmichael,2 and Canio Buonavoglia1

Department of Health and Animal Well-Being, Faculty of Veterinary Medicine, University of Bari, Bari, Italy,1 and James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York2

Received 27 November 2000/Returned for modification 23 January 2001/Accepted 23 February 2001

Canine parvovirus type 2 (CPV2) emerged in 1978 as causative agent of a new disease of dogs. New antigenic variants (biotypes), designated CPV2a and CPV2b, became widespread during 1979 to 1980 and 1984, respectively. At the present time the original CPV2 has disappeared in the dog population and has been replaced by the two new viruses. In the present study the comparison of neutralizing antibody titers in two groups of pups (18 pups in each group) inoculated with CPV2 and CPV2b modified live virus vaccines is reported. Using the hemagglutination inhibition (HI) test, relevant differences between antibody titers, against either the homologous or the heterologous virus, were not constantly observed. Using the neutralization (Nt) test, however, the pups inoculated with CPV2 had antibody titers which were approximately 30 times higher to the homologous virus (mean, 4,732) than to the heterologous virus (CPV2b) (mean, 162). The results of these experiments support two conclusions: (i) the HI test may not always accurately evaluate the true immune status of dogs with respect to CPV, and (ii) dogs inoculated with CPV2 vaccine develop relatively low Nt antibody titers against the heterologous virus (CPV2b). These data may suggest an advantage for new vaccines, considering that most presently licensed vaccines are produced with CPV2, which no longer exists in the dog population.

* Corresponding author. Mailing address: Department of Health and Animal Well-Being, Faculty of Veterinary Medicine, University of Bari, Strada per Casamassima km 3, 70010 Valenzano (Ba), Italy. Phone: 39-080-4679033. Fax: 39-080-4679043. E-mail: a.pratelli{at}veterinaria.uniba.it.

Clinical and Diagnostic Laboratory Immunology, May 2001, p. 612-615, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.612-615.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Decaro, N., Cirone, F., Desario, C., Elia, G., Lorusso, E., Colaianni, M. L., Martella, V., Buonavoglia, C. (2009). Severe parvovirus in a 12-year-old dog that had been repeatedly vaccinated. Vet Rec. 164: 593-595 [Full Text]  
  • Atkinson, M. (2006). Canine parvovirus vaccination. Vet Rec. 159: 895-895 [Full Text]  
  • Bergman, J. G. H. E. (2006). Canine parvovirus vaccination. Vet Rec. 159: 895-896 [Full Text]  
  • Martella, V., Cavalli, A., Decaro, N., Elia, G., Desario, C., Campolo, M., Bozzo, G., Tarsitano, E., Buonavoglia, C. (2005). Immunogenicity of an Intranasally Administered Modified Live Canine Parvovirus Type 2b Vaccine in Pups with Maternally Derived Antibodies. CVI 12: 1243-1245 [Abstract] [Full Text]  


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