This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ghanekar, S. A.
Right arrow Articles by Maino, V. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ghanekar, S. A.
Right arrow Articles by Maino, V. C.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, May 2001, p. 628-631, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.628-631.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Gamma Interferon Expression in CD8+ T Cells Is a Marker for Circulating Cytotoxic T Lymphocytes That Recognize an HLA A2-Restricted Epitope of Human Cytomegalovirus Phosphoprotein pp65

Smita A. Ghanekar,1,* Laurel E. Nomura,1 Maria A. Suni,1 Louis J. Picker,2 Holden T. Maecker,1 and Vernon C. Maino1

BD Biosciences, Immunocytometry Systems, San Jose, California 95131,1 and Vaccine and Gene Therapy Institute, Oregon Health Sciences University, Portland, Oregon 972012

Received 16 November 2000/Returned for modification 2 January 2001/Accepted 1 February 2001

Antigen-specific CD8+ T cells with cytotoxic activity are often critical in immune responses to infectious pathogens. To determine whether gamma interferon (IFN-gamma ) expression is a surrogate marker for cytotoxic T lymphocytes (CTL), human cytomegalovirus-specific CTL responses were correlated with CD8+ T-cell IFN-gamma expression determined by cytokine flow cytometry. A strong positive correlation was observed between specific lysis of peptide-pulsed targets in a 51Cr release assay and frequencies of peptide-activated CD8+ T cells expressing IFN-gamma at 6 h (r2 = 0.72) or 7 days (r2 = 0.91). Enumeration of responding cells expressing perforin, another marker associated with CTL, did not improve this correlation. These results demonstrate that IFN-gamma expression can be a functional surrogate for identification of CTL precursor cells.

* Corresponding author. Mailing address: BD Biosciences, 2350 Qume Dr., San Jose, CA 95131. Phone: (408) 954-4177. Fax: (408) 954-2156. E-mail: smita_ghanekar{at}bdis.com.

Clinical and Diagnostic Laboratory Immunology, May 2001, p. 628-631, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.628-631.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Milani, V., Frankenberger, B., Heinz, O., Brandl, A., Ruhland, S., Issels, R. D., Noessner, E. (2005). Melanoma-associated antigen tyrosinase but not Melan-A/MART-1 expression and presentation dissociate during the heat shock response. Int Immunol 17: 257-268 [Abstract] [Full Text]  
  • Mosolits, S., Markovic, K., Frodin, J.-E., Virving, L., Magnusson, C. G. M., Steinitz, M., Fagerberg, J., Mellstedt, H. (2004). Vaccination with Ep-CAM Protein or Anti-Idiotypic Antibody Induces Th1-Biased Response against MHC Class I- and II-Restricted Ep-CAM Epitopes in Colorectal Carcinoma Patients. Clin. Cancer Res. 10: 5391-5402 [Abstract] [Full Text]  
  • Hines, S. A., Stone, D. M., Hines, M. T., Alperin, D. C., Knowles, D. P., Norton, L. K., Hamilton, M. J., Davis, W. C., McGuire, T. C. (2003). Clearance of Virulent but Not Avirulent Rhodococcus equi from the Lungs of Adult Horses Is Associated with Intracytoplasmic Gamma Interferon Production by CD4+ and CD8+ T Lymphocytes. CVI 10: 208-215 [Abstract] [Full Text]  
  • Stevceva, L., Alvarez, X., Lackner, A. A., Tryniszewska, E., Kelsall, B., Nacsa, J., Tartaglia, J., Strober, W., Franchini, G. (2002). Both Mucosal and Systemic Routes of Immunization with the Live, Attenuated NYVAC/Simian Immunodeficiency Virus SIVgpe Recombinant Vaccine Result in Gag-Specific CD8+ T-Cell Responses in Mucosal Tissues of Macaques. J. Virol. 76: 11659-11676 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»