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Clinical and Diagnostic Laboratory Immunology, May 2001, p. 645-646, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.645-646.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Antibodies to Babesia equi in Horses by a Latex Agglutination Test Using Recombinant EMA-1

Xuenan Xuan,* Ikuo Igarashi, Tetsuya Tanaka, Shinya Fukumoto, Hideyuki Nagasawa, Kozo Fujisaki, and Takeshi Mikami

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

Received 17 November 2000/Returned for modification 22 January 2001/Accepted 2 March 2001

A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly between sera from B. equi-infected horses and sera from Babesia caballi-infected horses or from normal horses. The LAT results were identical to those of a previously developed enzyme-linked immunosorbent assay. These results indicate that LAT using recombinant EMA-1 might be very useful as a routine screening method for the diagnosis of B. equi infection.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5648. Fax: 81-155-49-5643. E-mail: gen{at}obihiro.ac.jp.

Clinical and Diagnostic Laboratory Immunology, May 2001, p. 645-646, Vol. 8, No. 3
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.3.645-646.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cunha, C. W., Kappmeyer, L. S., McGuire, T. C., Dellagostin, O. A., Knowles, D. P. (2002). Conformational Dependence and Conservation of an Immunodominant Epitope within the Babesia equi Erythrocyte-Stage Surface Protein Equi Merozoite Antigen 1. CVI 9: 1301-1306 [Abstract] [Full Text]  


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