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Clinical and Diagnostic Laboratory Immunology, July 2001, p. 768-771, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.768-771.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification and Strain Differentiation of Vibrio cholerae by Using Polyclonal Antibodies against Outer Membrane Proteins

A. Martínez-Govea,1 J. Ambrosio,2 L. Gutiérrez-Cogco,1 and A. Flisser1,2,*

Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Colonia Santo Tomás, 11340 DF,1 and Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, San Ángel 04510 DF,2 México

Received 11 January 2001/Returned for modification 27 March 2001/Accepted 17 April 2001

Cholera is caused only by O1 and O139 Vibrio cholerae strains. For diagnosis, 3 working days are needed for bacterial isolation from human feces and for biochemical characterization. Here we describe the purification of bacterial outer membrane proteins (OMP) from V. cholerae O1 Ogawa, O1 Inaba, and O139 strains, as well as the production of specific antisera and their use for fecal Vibrio antigen detection. Anti-OMP antisera showed very high reactivity and specificity by enzyme-linked immunosorbent assay (ELISA) and dot-ELISA. An inmunodiagnostic assay for V. cholerae detection was developed; this assay avoids preenrichment and costly equipment and can be used for epidemiological surveillance and clinical diagnosis of cases, considering that prompt and specific identification of bacteria is mandatory in cholera.

* Corresponding author. Mailing address: Departamento de Microbiología y Parasitología, Facultad de Medicina, Ciudad Universitaria, San Ángel 04510 DF, México. Phone: (525)6232466. Fax: (525)6232459. E-mail: flisser{at}servidor.unam.mx.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 768-771, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.768-771.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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