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Clinical and Diagnostic Laboratory Immunology, July 2001, p. 785-787, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.785-787.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Antenatal Screening for Hepatitis B and Antibodies to Toxoplasma gondii and Rubella Virus: Evaluation of Two Commercial Immunoassay Systems

Robert J. A. Diepersloot,1,* Hiske Dunnewold-Hoekstra,1 Jorien Kruit-den Hollander,1 and Fer Vlaspolder2

Laboratory for Medical Microbiology, Diakonessen Hospital, Utrecht,1 and Laboratory for Medical Microbiology, Alkmaar Medical Centre, Alkmaar,2 The Netherlands

Received 11 September 2000/Returned for modification 19 December 2000/Accepted 27 March 2001

A comparative evaluation of the Abbott AxSYM and DPC Immulite random-access analyzers was performed using 497 prospectively collected serum samples. These samples were sent to the laboratory for routine antenatal screening for hepatitis B surface antigen and immunoglobulin G (IgG) and IgM antibodies to Toxoplasma gondii and rubella virus. The overall agreement between the two assay systems ranged from 97.4 to 100%. After discrepancy analysis, the outcome in terms of sensitivity and specificity varied from 98.2 to 100% for all but one of the assays tested. The AxSYM rubella virus IgG assay tended to report protective or indeterminate antibody levels in 1% of the samples. This shortcoming might be overcome by raising the cutoff of the microparticle enzyme immunoassay system.

* Corresponding author. Mailing address: Laboratory for Medical Microbiology and Immunology, Diakonessen Hospital, Bosboomstraat 1, 3582 KE Utrecht, The Netherlands. Phone: 00-31302566518. Fax: 00-31302566695. E-mail: Rdiepersloot{at}diakhuis.nl.

Clinical and Diagnostic Laboratory Immunology, July 2001, p. 785-787, Vol. 8, No. 4
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.4.785-787.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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