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Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1056-1059, Vol. 8, No. 6
Department of Bacteriology, Okayama
University Medical School, 2-5-1 Shikata-cho Okayama,
700-8558,1 Department of
Gastroenterology, Hokkaido University Graduate School of Medicine, N15,
W7, Kita-ku, Sapporo, 060-0815,2 and
Department of Microbiology, Jichi Medical School, 3311-1 Yakushiji Minamikouch-cho Kawauch-Gun,
329-0498,3 Japan
Received 5 April 2001/Returned for modification 24 May
2001/Accepted 23 July 2001
Gastric mucosa-associated lymphoid tissue (MALT) lymphoma is
related to Helicobacter pylori infection. Specifically, it
has been pointed out that pathogenesis of MALT lymphoma involves the 60-kDa heat shock protein (hsp60). To investigate humoral immune responses to the H. pylori hsp60 in patients with
gastroduodenal diseases and patients with MALT lymphoma, the hsp60 of
H. pylori was expressed with a glutathione
S-transferase fusion protein and was purified
(recombinant hsp60). Sera were obtained from H. pylori-positive patients with gastroduodenal diseases (MALT lymphoma, n = 13; gastric ulcer, n = 20; duodenal ulcer, n = 20; gastritis,
n = 20) and from H. pylori-negative
healthy volunteers (n = 9). Sera from patients with
MALT lymphoma were also obtained at two times: before and after
eradication therapy. Antibodies to hsp60 and H. pylori were
assessed by enzyme-linked immunosorbent assay. The levels of
immunoglobulin G (IgG) antibodies to the hsp60 of H. pylori-positive patients with gastroduodenal diseases were
significantly elevated compared to those in the controls. The levels of
IgG1 antibodies to hsp60 were elevated and correlated with the levels
of anti-H. pylori antibodies in patients with MALT
lymphoma. Humarol immunity against hsp60 may be important and relevant
to gastroduodenal diseases induced by H. pylori infection.
1071-412X/01/$04.00+0 DOI: 10.1128/CDLI.8.6.1056-1059.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Immunoglobulin G1 Antibody Response to
Helicobacter pylori Heat Shock Protein 60 Is Closely
Associated with Low-Grade Gastric Mucosa-Associated Lymphoid
Tissue Lymphoma
*
Corresponding author. Mailing address: Department of
Bacteriology, Okayama University Medical School, 2-5-1 Shikata-cho
Okayama, 700-8558, Japan. Phone and fax: 81-86-235-7162. E-mail:
kuma{at}med.okayama-u.ac.jp.
Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1056-1059, Vol. 8, No. 6
1071-412X/01/$04.00+0 DOI: 10.1128/CDLI.8.6.1056-1059.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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