This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zmuda, J. F.
Right arrow Articles by Whiteley, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zmuda, J. F.
Right arrow Articles by Whiteley, G.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1267-1270, Vol. 8, No. 6
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.6.1267-1270.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Recognition of Multiple Classes of Hepatitis C Antibodies Increases Detection Sensitivity in Oral Fluid

Jonathan F. Zmuda,* Barbara Wagoneer, Lance Liotta, and Gordon Whiteley

Immunomatrix Inc., Gaithersburg, Maryland

Received 11 May 2001/Returned for modification 12 July 2001/Accepted 26 July 2001

Paired serum-oral fluid samples from 127 hepatitis C virus (HCV)-positive and 31 HCV-negative patients were tested for the presence of anti-HCV using the Ortho HCV 3.0 ELISA. Using the immunoglobulin G (IgG)-specific detection antibody provided with the HCV 3.0 ELISA we attained 100% sensitivity and specificity with serum samples; however, sensitivity in oral fluid samples was only 81%. By modifying the HCV 3.0 ELISA to utilize a secondary antibody cocktail that recognizes not only IgG but IgA and IgM as well, we attained 100% specificity and sensitivity with oral fluid samples.

* Corresponding author. Mailing address: Immunomatrix Inc., Gaithersburg, MD 20878. Phone: (301) 208-8000. Fax: (301) 208-9731. E-mail: JonZmuda{at}clark.net.

Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1267-1270, Vol. 8, No. 6
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.6.1267-1270.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»