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Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1271-1276, Vol. 8, No. 6
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.6.1271-1276.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Analogs of Eap Protein Are Conserved and Prevalent in Clinical Staphylococcus aureus Isolates

Muzaffar Hussain,* Karsten Becker, Christof von Eiff, Georg Peters, and Mathias Herrmann

Institute of Medical Microbiology, University of Muenster, Muenster, Germany

Received 19 March 2001/Returned for modification 5 June 2001/Accepted 23 July 2001

Map and Eap are secreted Staphylococcus aureus proteins that interact with various extracellular matrix molecules. PCR analysis using map primers yielded positive reactions in 97.9% of S. aureus isolates but not in Staphylococcus epidermidis isolates. Cloning and sequencing of the conferring genes revealed a high degree of overall homology combined with size variability of the gene product due to various repeat numbers and early translation termination in a poly(A) region. Thus, Map and Eap may provide a potential novel tool for S. aureus identification and typing.

* Corresponding author. Mailing address: Institute of Medical Microbiology, University Hospital, Domagkstr. 10, 48129 Muenster, Germany. Phone: 49-251-835 5345. Fax: 49-251-835 5350. E-mail: muzaffa{at}uni-muenster.de.

Clinical and Diagnostic Laboratory Immunology, November 2001, p. 1271-1276, Vol. 8, No. 6
1071-412X/01/$04.00+0   DOI: 10.1128/CDLI.8.6.1271-1276.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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