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Clinical and Diagnostic Laboratory Immunology, January 2002, p. 190-193, Vol. 9, No. 1
1071-412X/01/$04.00+0     DOI: 10.1128/CDLI.9.1.190-193.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium)

Alessandra Xavier Pardini,1 Regina Helena Peralta,2 Adelaide José Vaz,1* Luis dos Ramos Machado,3 and José Mauro Peralta2

Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences,1 Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil,2 Center for Neurological Investigation, Faculty of Medicine, University of São Paulo, São Paulo3

Received 7 May 2001/ Returned for modification 13 August 2001/ Accepted 9 October 2001

Antigen extracts obtained from the vesicular fluid of Taenia crassiceps cysticerci and from fractions purified by affinity chromatography with the lectin concanavalin A and the glycoprotein antigen separated by electrophoresis were used for the detection of Taenia solium anticysticercus antibodies. The sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. Using immunoblotting of the three antigens, low-molecular-mass peptides (18 and 14 kDa) were characterized only in cerebrospinal fluid samples from patients with neurocysticercosis. The results confirm that antigen fractions purified from T. crassiceps cisticerci are important sources of specific peptides and proved to be efficient in detecting anti-T. solium antibodies.

* Corresponding author. Mailing address: Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, Bloco 17, CEP 05508-90, São Paulo SP, Brazil. Phone: 55 11 8183638. Fax: 55 11 8132197. E-mail: ajvaz{at}netpoint.com.br.

Clinical and Diagnostic Laboratory Immunology, January 2002, p. 190-193, Vol. 9, No. 1
1071-412X/01/$04.00+0     DOI: 10.1128/CDLI.9.1.190-193.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Espindola, N. M., Iha, A. H., Fernandes, I., Takayanagui, O. M., Machado, L. d. R., Livramento, J. A., Mendes Maia, A. A., Peralta, J. M., Vaz, A. J. (2005). Cysticercosis Immunodiagnosis Using 18- and 14-Kilodalton Proteins from Taenia crassiceps Cysticercus Antigens Obtained by Immunoaffinity Chromatography. J. Clin. Microbiol. 43: 3178-3184 [Abstract] [Full Text]  


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