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Clinical and Diagnostic Laboratory Immunology, January 2002, p. 198-200, Vol. 9, No. 1
1071-412X/01/$04.00+0     DOI: 10.1128/CDLI.9.1.198-200.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Dengue Virus Replicative Intermediate RNA Detection by Reverse Transcription-PCR

Gilberto Vaughan,1 Hiram Olivera,1 Leopoldo Santos-Argumedo,2 Abraham Landa,3 Baltasar Briseño,1 and Alejandro Escobar-Gutiérrez1*

Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, México DF 11340,1 Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México DF 07000,2 Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF 04510, México3

Received 27 April 2001/ Returned for modification 15 August 2001/ Accepted 29 October 2001

Dengue virus replication involves synthesis of a replicative intermediate RNA (RI-RNA), whose presence reveals an actual infection. We report on a simple and rapid reverse transcription-PCR for the detection of viral RI-RNA in infected cells. The product is demonstrated at 20 min postinfection. This method is useful for the study of virus-cell tropism.

* Corresponding author. Mailing address: Departamento de Investigaciones Inmunológicas, Instituto de Diagnóstico y Referencia Epidemiológicos, Carpio 470, Col. Santo Tomas, México DF 11340, México. Phone: (525) 341-1833. Fax: (525) 341-3264. E-mail: aescobaa{at}conacyt.mx.

Clinical and Diagnostic Laboratory Immunology, January 2002, p. 198-200, Vol. 9, No. 1
1071-412X/01/$04.00+0     DOI: 10.1128/CDLI.9.1.198-200.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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