This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martin, D. A.
Right arrow Articles by Roehrig, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martin, D. A.
Right arrow Articles by Roehrig, J. T.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, May 2002, p. 544-549, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.544-549.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of Immunoglobulin M Cross-Reactions in Differential Diagnosis of Human Flaviviral Encephalitis Infections in the United States

Denise A. Martin,* Brad J. Biggerstaff, Becky Allen, Alison J. Johnson, Robert S. Lanciotti, and John T. Roehrig

Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522

Received 19 October 2001/ Returned for modification 31 December 2001/ Accepted 15 January 2002

To define the virus specificity of the immunoglobulin M (IgM) antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) among the medically important members of the Japanese encephalitis (JE) virus serocomplex of flaviviruses, 103 IgM-positive human serum samples from patients with confirmed West Nile (WN) virus, St. Louis encephalitis (SLE) virus, or JE virus infections were assembled and simultaneously tested against all three viral antigens in a standardized MAC-ELISA. Of the serum samples tested, 96 (93%) showed higher positive-to-negative absorbance ratios (P/Ns) with the infecting virus antigen compared to those obtained with the other two virus antigens. Of the seven specimens with higher P/Ns with heterologous virus antigens, six were from patients with SLE virus infections (the serum samples had higher levels of reactivity with WN virus antigen) and one was from a patient with a JE virus infection (this serum sample also had a higher level of reactivity with WN virus antigen). Not surprisingly, similar virus specificity was observed with WN virus-elicited IgM in cerebrospinal fluid. As shown in previous studies, a subset of these specimens was even less reactive in the MAC-ELISA with dengue virus, a member of a different flavivirus serocomplex. The degree of virus cross-reactivity did not appear to be related to days postonset, at least during the first 40 days of infection. Infections with WN virus could be correctly distinguished from infections with SLE virus on the basis of the observed anti-viral IgM cross-reactivities alone 92% of the time. Infections with SLE virus resulted in antibody that was more cross-reactive, so identification of SLE virus as the infecting agent by use of MAC-ELISA cross-reactivity alone was more problematic.

* Corresponding author. Mailing address: Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Fort Collins, CO 80522. Phone: (970) 221-6445. Fax: (970) 221-6476. E-mail: DZM9{at}CDC.GOV.

Clinical and Diagnostic Laboratory Immunology, May 2002, p. 544-549, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.544-549.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Loeb, M., Hanna, S., Nicolle, L., Eyles, J., Elliott, S., Rathbone, M., Drebot, M., Neupane, B., Fearon, M., Mahony, J. (2008). Prognosis after West Nile Virus Infection. ANN INTERN MED 149: 232-241 [Abstract] [Full Text]  
  • Johnson, B. W., Russell, B. J., Lanciotti, R. S. (2005). Serotype-Specific Detection of Dengue Viruses in a Fourplex Real-Time Reverse Transcriptase PCR Assay. J. Clin. Microbiol. 43: 4977-4983 [Abstract] [Full Text]  
  • Tilley, P. A. G., Walle, R., Chow, A., Jayaraman, G. C., Fonseca, K., Drebot, M. A., Preiksaitis, J., Fox, J. (2005). Clinical Utility of Commercial Enzyme Immunoassays during the Inaugural Season of West Nile Virus Activity, Alberta, Canada. J. Clin. Microbiol. 43: 4691-4695 [Abstract] [Full Text]  
  • Vinayagamoorthy, T., Mulatz, K., Drebot, M., Hodkinson, R. (2005). Molecular Typing of West Nile Virus, Dengue, and St. Louis Encephalitis Using Multiplex Sequencing. J. Mol. Diagn. 7: 152-159 [Abstract] [Full Text]  
  • Taylor, C., Simmons, R., Smith, I. (2005). Development of Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay To Differentiate Human Flavivirus Infections Occurring in Australia. CVI 12: 371-374 [Abstract] [Full Text]  
  • Martin, D. A., Noga, A., Kosoy, O., Johnson, A. J., Petersen, L. R., Lanciotti, R. S. (2004). Evaluation of a Diagnostic Algorithm Using Immunoglobulin M Enzyme-Linked Immunosorbent Assay To Differentiate Human West Nile Virus and St. Louis Encephalitis Virus Infections during the 2002 West Nile Virus Epidemic in the United States. CVI 11: 1130-1133 [Abstract] [Full Text]  
  • PUGACHEV, K. V., GUIRAKHOO, F., MITCHELL, F., OCRAN, S. W., PARSONS, M., JOHNSON, B. W., KOSOY, O. L., LANCIOTTI, R. S., ROEHRIG, J. T., TRENT, D. W., MONATH, T. P. (2004). CONSTRUCTION OF YELLOW FEVER/ST. LOUIS ENCEPHALITIS CHIMERIC VIRUS AND THE USE OF CHIMERAS AS A DIAGNOSTIC TOOL. Am J Trop Med Hyg 71: 639-645 [Abstract] [Full Text]  
  • Hogrefe, W. R., Moore, R., Lape-Nixon, M., Wagner, M., Prince, H. E. (2004). Performance of Immunoglobulin G (IgG) and IgM Enzyme-Linked Immunosorbent Assays Using a West Nile Virus Recombinant Antigen (preM/E) for Detection of West Nile Virus- and Other Flavivirus-Specific Antibodies. J. Clin. Microbiol. 42: 4641-4648 [Abstract] [Full Text]  
  • Purdy, D. E., Noga, A. J., Chang, G.-J. J. (2004). Noninfectious Recombinant Antigen for Detection of St. Louis Encephalitis Virus-Specific Antibodies in Serum by Enzyme-Linked Immunosorbent Assay. J. Clin. Microbiol. 42: 4709-4717 [Abstract] [Full Text]  
  • Shu, P.-Y., Huang, J.-H. (2004). Current Advances in Dengue Diagnosis. CVI 11: 642-650 [Full Text]  
  • Muerhoff, A. S., Dawson, G. J., Dille, B., Gutierrez, R., Leary, T. P., Gupta, M. C., Kyrk, C. R., Kapoor, H., Clark, P., Schochetman, G., Desai, S. M. (2004). Enzyme-Linked Immunosorbent Assays Using Recombinant Envelope Protein Expressed in COS-1 and Drosophila S2 Cells for Detection of West Nile Virus Immunoglobulin M in Serum or Cerebrospinal Fluid. CVI 11: 651-657 [Abstract] [Full Text]  
  • Shu, P.-Y., Chen, L.-K., Chang, S.-F., Su, C.-L., Chien, L.-J., Chin, C., Lin, T.-H., Huang, J.-H. (2004). Dengue Virus Serotyping Based on Envelope and Membrane and Nonstructural Protein NS1 Serotype-Specific Capture Immunoglobulin M Enzyme-Linked Immunosorbent Assays. J. Clin. Microbiol. 42: 2489-2494 [Abstract] [Full Text]  
  • Beasley, D. W. C., Holbrook, M. R., Travassos da Rosa, A. P. A., Coffey, L., Carrara, A.-S., Phillippi-Falkenstein, K., Bohm, R. P. Jr., Ratterree, M. S., Lillibridge, K. M., Ludwig, G. V., Estrada-Franco, J., Weaver, S. C., Tesh, R. B., Shope, R. E., Barrett, A. D. T. (2004). Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection. J. Clin. Microbiol. 42: 2759-2765 [Abstract] [Full Text]  
  • Gea-Banacloche, J., Johnson, R. T., Bagic, A., Butman, J. A., Murray, P. R., Agrawal, A. G. (2004). West Nile Virus: Pathogenesis and Therapeutic Options. ANN INTERN MED 140: 545-553 [Abstract] [Full Text]  
  • Prince, H. E., Lape'-Nixon, M., Moore, R. J., Hogrefe, W. R. (2004). Utility of the Focus Technologies West Nile Virus Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for Testing Cerebrospinal Fluid. J. Clin. Microbiol. 42: 12-15 [Abstract] [Full Text]  
  • Prince, H. E., Hogrefe, W. R. (2003). Detection of West Nile Virus (WNV)-Specific Immunoglobulin M in a Reference Laboratory Setting during the 2002 WNV Season in the United States. CVI 10: 764-768 [Abstract] [Full Text]  
  • Wong, S. J., Boyle, R. H., Demarest, V. L., Woodmansee, A. N., Kramer, L. D., Li, H., Drebot, M., Koski, R. A., Fikrig, E., Martin, D. A., Shi, P.-Y. (2003). Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination. J. Clin. Microbiol. 41: 4217-4223 [Abstract] [Full Text]  
  • Sejvar, J. J., Haddad, M. B., Tierney, B. C., Campbell, G. L., Marfin, A. A., Van Gerpen, J. A., Fleischauer, A., Leis, A. A., Stokic, D. S., Petersen, L. R. (2003). Neurologic Manifestations and Outcome of West Nile Virus Infection. JAMA 290: 511-515 [Abstract] [Full Text]  
  • Solomon, T., Ooi, M. H., Beasley, D. W C, Mallewa, M. (2003). West Nile encephalitis. BMJ 326: 865-869 [Full Text]  
  • Madden, K. (2003). West Nile Virus Infection and its Neurological Manifestations. Clin Med Res 1: 145-150 [Abstract] [Full Text]  
  • Bains, H. S., Jampol, L. M., Caughron, M. C., Parnell, J. R. (2003). Vitritis and Chorioretinitis in a Patient With West Nile Virus Infection. Arch Ophthalmol 121: 205-207 [Abstract] [Full Text]  
  • Prince, H. E., Hogrefe, W. R. (2003). Performance Characteristics of an In-House Assay System Used To Detect West Nile Virus (WNV)-Specific Immunoglobulin M during the 2001 WNV Season in the United States. CVI 10: 177-179 [Abstract] [Full Text]  
  • Petersen, L. R., Marfin, A. A. (2002). West Nile Virus: A Primer for the Clinician. ANN INTERN MED 137: 173-179 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»