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Clinical and Diagnostic Laboratory Immunology, May 2002, p. 627-632, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.627-632.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cloning and Expression of a Helicobacter bilis Immunoreactive Protein

Sunlian Feng, Emir Hodzic, Lon V. Kendall, Amy Smith, Kimberly Freet, and Stephen W. Barthold*

Center for Comparative Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California

Received 2 November 2001/ Returned for modification 18 December 2001/ Accepted 16 January 2002

In an effort to identify immunoreactive Helicobacter bilis antigens with potential for serodiagnosis, sera from mice experimentally infected with H. bilis were used to screen an H. bilis genomic DNA expression library. Among 17 immunoreactive clones, several contained sequences that encoded a predicted 167-kDa protein (P167). Five overlapping P167 peptides (P167A to P167E) of approximately 40 kDa each were generated and tested. Immune sera reacted with fragments P167C and P167D at dilutions of 1:1,600 and 1:6,400, respectively, and reacted with an H. bilis membrane extract at a dilution of 1:800 in an enzyme-linked immunosorbent assay. Sera from mice experimentally infected with H. hepaticus did not react with P167C and P167D. Sera from mice naturally infected with H. bilis but not sera from mice naturally infected with H. hepaticus reacted with P167C and P167D. Hyperimmune sera against P167C peptide reacted with recombinant P167C and with a 120-kDa band in H. bilis lysates but did not react with a protein of the same size on immunoblots prepared from H. hepaticus, H. muridarum, or unrelated Borrelia burgdorferi and Campylobacter jejuni whole-cell lysates. Nevertheless, the P167A, P167B, P167C, and P167D primers, but not the P167E primers, amplified DNA from H. hepaticus, and all five primer sets amplified DNA from H. muridarum. These results suggest that P167 is an immunodominant, H. bilis-specific antigen that may have potential for use in serodiagnosis.

* Corresponding author. Mailing address: Center for Comparative Medicine, University of California at Davis, One Shields Ave., Davis, CA 95616. Phone: (530) 752-1245. Fax: (530) 752-7914. E-mail: swbarthold{at}ucdavis.edu.

Clinical and Diagnostic Laboratory Immunology, May 2002, p. 627-632, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.627-632.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Feng, S., Ku, K., Hodzic, E., Lorenzana, E., Freet, K., Barthold, S. W. (2005). Differential Detection of Five Mouse-Infecting Helicobacter Species by Multiplex PCR. CVI 12: 531-536 [Abstract] [Full Text]  
  • Feng, S., Kendall, L. V., Hodzic, E., Wong, S., Lorenzana, E., Freet, K., Ku, K. S., Luciw, P. A., Barthold, S. W., Khan, I. H. (2004). Recombinant Helicobacter bilis Protein P167 for Mouse Serodiagnosis in a Multiplex Microbead Assay. CVI 11: 1094-1099 [Abstract] [Full Text]  


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