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Clinical and Diagnostic Laboratory Immunology, May 2002, p. 708-712, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.708-712.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effect of Contaminating Red Blood Cells on OKT3-Mediated Polyclonal Activation of Peripheral Blood Mononuclear Cells

Samuel Song,1,2,{dagger} Joseph Goodwin,1,{ddagger} Jenny Zhang,1,§ Bruce Babbitt,1,|| and Janet L. Lathey2*

Research Department, Cellcor Inc., Newton, Massachusetts 02139,1 Zycos, Inc., Lexington, Massachusetts 024212

Received 7 September 2001/ Returned for modification 23 October 2001/ Accepted 4 March 2002

Erythrocytes are typically present as impurities in the majority of peripheral blood mononuclear cell (PBMC) preparations. This study was undertaken to investigate the effects of contaminating red blood cells (RBC) on the ability of OKT3 to activate CD4+ and CD8+ T cells. Surprisingly, the levels of gamma interferon, tumor necrosis factor alpha, and interleukin-1ß (IL-1ß) produced by PBMC upon stimulation by OKT3 were increased (P < 0.05) in a dose-dependent manner when increasing amounts of autologous RBC (RBC-to-PBMC ratios of 2:1, 10:1, and 50:1) were spiked into PBMC preparations. The OKT3-driven induction of the IL-2 receptor (CD25) and the proliferation of T lymphocytes in response to phorbol myristate acetate were not affected by the addition of RBC.

* Corresponding author. Present address: 44 Hartwell Ave., Lexington, MA 02421. Phone: (781) 274-6500, ext. 234. Fax: (781) 274-0839. E-mail: jlathey{at}hotmail.com.

{dagger} Present address: Biotransplant, Medford, Mass.

{ddagger} Present address: BD Biosciences, Bedford, Mass.

§ Present address: Purdue Pharma L.P., Princeton, N.J.

|| Present address: Parexel, Waltham, Mass.

Clinical and Diagnostic Laboratory Immunology, May 2002, p. 708-712, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.708-712.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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