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Clinical and Diagnostic Laboratory Immunology, May 2002, p. 716-719, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.716-719.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

One-Step PCR To Distinguish B Virus from Related Primate Alphaherpesviruses

Makoto Hirano,1 Shin Nakamura,1* Fusako Mitsunaga,1 Maki Okada,1 Shuya Shirahama,2 and Richard Eberle3

Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506,1 Gene Analysis Section, Gene and Chromosome Analysis Center, Hachioji Laboratory, SRL Co., Ltd., Hachioji, Tokyo 192-8535, Japan,2 Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078-20073

Received 30 August 2001/ Returned for modification 11 December 2001/ Accepted 10 January 2002

By adding betaine to the PCR mixture, we previously established a PCR method to amplify a DNA segment of the glycoprotein G gene of B virus (BV) derived from a rhesus macaque. We have found that DNA of other BV strains derived from cynomolgus, pigtail, and lion-tailed macaques can also serve as the template in our PCR assay. Under the same conditions no product was obtained with DNA of simian agent 8 of green monkeys and Herpesvirus papio 2 of baboons, or the human herpes simplex viruses types 1 and 2. Thus, this PCR method is useful to discriminate BV from other closely related primate alphaherpesviruses.

* Corresponding author. Mailing address: Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan. Phone: 81(0)568-63-0579. Fax: 81(0)568-63-0579. E-mail: snakamur{at}pri.kyoto-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, May 2002, p. 716-719, Vol. 9, No. 3
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.3.716-719.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Luebcke, E., Dubovi, E., Black, D., Ohsawa, K., Eberle, R. (2006). Isolation and characterization of a chimpanzee alphaherpesvirus. J. Gen. Virol. 87: 11-19 [Abstract] [Full Text]  


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