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Clinical and Diagnostic Laboratory Immunology, July 2002, p. 808-811, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.808-811.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Antigenicity of Leishmania braziliensis Histone H1 during Cutaneous Leishmaniasis: Localization of Antigenic Determinants

Emma Carmelo,1 Enrique Martínez,1 Ana Cristina González,1 José Enrique Piñero,1 Manuel E. Patarroyo,2 Antonio del Castillo,1 and Basilio Valladares1*

Departamento de Parasitología, Facultad de Farmacia, Universidad de La Laguna, La Laguna, S/C de Tenerife, Spain,1 Instituto de Inmunología San Juan de Dios, Bogotá, Colombia2

Received 10 December 2001/ Returned for modification 5 March 2002/ Accepted 6 April 2002

The humoral immune response against Leishmania braziliensis histone H1 by patients with cutaneous leishmaniasis is described. For this purpose, the protein was purified as a recombinant protein in a prokaryotic expression system and was assayed by enzyme-linked immunosorbent assay (ELISA) with a collection of sera from patients with cutaneous leishmaniasis and Chagas' disease. The assays showed that L. braziliensis histone H1 was recognized by 66% of the serum samples from patients with leishmaniasis and by 40% of the serum samples from patients with Chagas' disease, indicating that it acts as an immunogen during cutaneous leishmaniasis. In order to locate the linear antigenic determinants of this protein, a collection of synthetic peptides covering the L. braziliensis histone H1sequence was tested by ELISA. The experiments showed that the main antigenic determinant is located in the central region of this protein. Our results show that the recombinant L. braziliensis histone H1 is recognized by a significant percentage of serum samples from patients with cutaneous leishmaniasis, but use of this protein as a tool for the diagnosis of cutaneous leishmaniasis is hampered by the cross-reaction with sera from patients with Chagas' disease.

* Corresponding author. Mailing address: Departamento de Parasitología, Facultad de Farmacia, Universidad de La Laguna, Ast. Fco. Sánchez, s/n 38204 La Laguna, S/C de Tenerife, Spain. Phone: 34 922 318430. Fax: 34 922 318514. E-mail: bvallada{at}ull.es.

Clinical and Diagnostic Laboratory Immunology, July 2002, p. 808-811, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.808-811.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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