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Clinical and Diagnostic Laboratory Immunology, July 2002, p. 858-863, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.858-863.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

MaGiK Method of T-Cell Receptor Repertoire Analysis

M. Scott Killian,1,2* Jose Matud,2,3 Roger Detels,1 Janis V. Giorgi,2,{dagger} and Beth D. Jamieson2

Department of Epidemiology,1 Department of Medicine and Jonsson Comprehensive Cancer Center, and,2 Department of Biostatistics,University of California, Los Angeles, California3

Received 14 November 2001/ Returned for modification 29 January 2002/ Accepted 10 April 2002

T-cell receptor diversity enables the cellular immune response to recognize a broad range of viral and other pathogenic agents. An increasingly common method of characterizing T-cell receptor diversity and usage in response to antigenic challenges involves the identification of clonal expansions by PCR amplification of the CDR3 region of distinct TCRVß families. Though clonal expansions often appear evident upon visual inspection of the results, a systematic method is needed for the valid enumeration of these expansions. Here, we describe a novel analysis method, termed the MaGiK method, for systematically identifying and enumerating clonal T-cell expansions and for applying the results to investigations of the T-cell receptor repertoire.

* Corresponding author. Mailing address: UCLA School of Medicine/Cellular Immunology and Cytometry, 12-236 Factor Bldg., Los Angeles, CA 90095-1745. Phone: (310) 825-4937. Fax: (310) 206-7854. E-mail: skill{at}ucla.edu.

{dagger} Deceased May 30, 2000.

Clinical and Diagnostic Laboratory Immunology, July 2002, p. 858-863, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.858-863.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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