This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pickering, J. W.
Right arrow Articles by Hill, H. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pickering, J. W.
Right arrow Articles by Hill, H. R.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, July 2002, p. 872-876, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.872-876.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of a Multiplex Flow Cytometric Assay with Enzyme-Linked Immunosorbent Assay for Quantitation of Antibodies to Tetanus, Diphtheria, and Haemophilus influenzae Type b

Jerry W. Pickering,1* Thomas B. Martins,1 M. Carl Schroder,1 and Harry R. Hill1,2

Associated Regional and University Pathologists, Institute for Clinical and Experimental Pathology,1 Department of Pathology, Pediatrics and Medicine, University of Utah School of Medicine, Salt Lake City, Utah 841082

Received 11 February 2002/ Returned for modification 20 March 2002/ Accepted 24 April 2002

We developed a multiplexed indirect immunofluorescence assay for antibodies to Haemophilus influenza type b (Hib) polysaccharide and the toxoids of Clostridium tetani (Tet) and Corynebacterium diphtheriae (Dip) based on the Luminex multiple-analyte profiling system. A pooled serum standard was calibrated against World Health Organization standards for Dip and Tet and an international standard for Hib. The multiplexed Luminex assay was compared to individual enzyme-linked immunosorbent assays (ELISAs) for the same analytes. By both methods, 75 (92.6%) of 81 of random serum samples had protective levels of antibody to Tet (>=0.1 IU/ml). For Dip, 81.5% of the samples had protective antibody levels (>=0.1 IU/ml) by ELISA and 80.2% had protective antibody levels by Luminex. Protective levels (>=1.0 µg/ml) of antibody to Hib were found in 45.0% of the samples tested by ELISA and in 39.0% of the samples tested by Luminex. The correlations (R2) between ELISA and Luminex of the 81 samples were 0.96, 0.96, and 0.91 for Tet, Dip, and Hib, respectively. There was also similar agreement between Luminex and ELISA for sera collected before and 1 month after Tet, Dip, and Hib vaccine administration. Both methods detected strong postvaccination responses. The Luminex method is an attractive alternative to ELISA since it reduces labor and reagent costs, as well as assay time.

* Corresponding author. Mailing address: ARUP Institute, 500 Chipeta Way, Salt Lake City, UT 84108. Phone: (801) 583-2787. Fax: (801) 583-2712. E-mail: pickerjw{at}aruplab.com.

Clinical and Diagnostic Laboratory Immunology, July 2002, p. 872-876, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.872-876.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Khan, I. H., Mendoza, S., Yee, J., Deane, M., Venkateswaran, K., Zhou, S. S., Barry, P. A., Lerche, N. W., Luciw, P. A. (2006). Simultaneous Detection of Antibodies to Six Nonhuman-Primate Viruses by Multiplex Microbead Immunoassay. CVI 13: 45-52 [Abstract] [Full Text]  
  • Gannot, G., Tangrea, M. A., Gillespie, J. W., Erickson, H. S., Wallis, B. S., Leakan, R. A., Knezevic, V., Hartmann, D. P., Chuaqui, R. F., Emmert-Buck, M. R. (2005). Layered Peptide Arrays: High-Throughput Antibody Screening of Clinical Samples. J. Mol. Diagn. 7: 427-436 [Abstract] [Full Text]  
  • Waterboer, T., Sehr, P., Michael, K. M., Franceschi, S., Nieland, J. D., Joos, T. O., Templin, M. F., Pawlita, M. (2005). Multiplex Human Papillomavirus Serology Based on In Situ-Purified Glutathione S-Transferase Fusion Proteins. Clin. Chem. 51: 1845-1853 [Abstract] [Full Text]  
  • Khan, I. H., Kendall, L. V., Ziman, M., Wong, S., Mendoza, S., Fahey, J., Griffey, S. M., Barthold, S. W., Luciw, P. A. (2005). Simultaneous Serodetection of 10 Highly Prevalent Mouse Infectious Pathogens in a Single Reaction by Multiplex Analysis. CVI 12: 513-519 [Abstract] [Full Text]  
  • Martins, T. B., Burlingame, R., von Muhlen, C. A., Jaskowski, T. D., Litwin, C. M., Hill, H. R. (2004). Evaluation of Multiplexed Fluorescent Microsphere Immunoassay for Detection of Autoantibodies to Nuclear Antigens. CVI 11: 1054-1059 [Abstract] [Full Text]  
  • Lal, G., Balmer, P., Joseph, H., Dawson, M., Borrow, R. (2004). Development and Evaluation of a Tetraplex Flow Cytometric Assay for Quantitation of Serum Antibodies to Neisseria meningitidis Serogroups A, C, Y, and W-135. CVI 11: 272-279 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»