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Clinical and Diagnostic Laboratory Immunology, July 2002, p. 898-900, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.898-900.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Screening of Neonatal Calves for Persistent Infection with Bovine Viral Diarrhea Virus by Immunohistochemistry on Skin Biopsy Samples

Daniel L. Grooms* and Eric D. Keilen

Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, Michigan 48895

Received 2 January 2002/ Returned for modification 11 March 2002/ Accepted 8 April 2002

Detection and elimination of cattle that are persistently infected with bovine viral diarrhea virus (BVDV) is important for controlling the transmission of this virus. Colostrum-derived antibodies make the detection of persistently BVDV-infected neonatal calves cumbersome and expensive. The objective of this study was to evaluate the use of immunohistochemical staining of skin biopsy samples from neonatal calves as a method for the early detection of persistent BVDV infection. Three hundred thirty-two 1- to 4-week-old dairy calves were screened for BVDV as part of a routine control program. Formalin-fixed skin biopsy samples were stained for BVDV antigen by immunohistochemistry (IHC), and the results were compared to those of virus isolation (VI) from white blood cell preparations. Six calves were positive by both IHC and VI and remained positive for BVDV upon subsequent follow-up testing; thus, they were classified as persistently infected with BVDV. One calf was positive by VI but negative by IHC. On subsequent testing, the calf was negative by VI, suggesting that the initial VI result was due to an acute BVDV infection. One calf was positive by IHC but negative by VI. This calf remained negative by VI on follow-up testing. Immunohistochemical staining of skin biopsy samples is a reliable method for screening neonatal calves for persistent BVDV infection and would be a useful management tool as an aid for controlling and preventing BVDV infection.

* Corresponding author. Mailing address: Michigan State University, College of Veterinary Medicine, Department of Large Animal Clinical Sciences, A100 VTH, East Lansing, MI 48824. Phone: (517) 432-1494. Fax: (517) 432-1042. E-mail: groomsd{at}cvm.msu.edu.

Clinical and Diagnostic Laboratory Immunology, July 2002, p. 898-900, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.898-900.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schefers, J., Munoz-Zanzi, C., Collins, J. E., Goyal, S. M., Ames, T. R. (2008). Serological evaluation of precolostral serum samples to detect Bovine viral diarrhea virus infections in large commercial dairy herds. jvdi 20: 625-628 [Abstract] [Full Text]  


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