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Clinical and Diagnostic Laboratory Immunology, July 2002, p. 935-937, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.935-937.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Interleukin-8 and Tumor Necrosis Factor Alpha Production in Human Epidermal Keratinocytes Induced by Trichophyton mentagrophytes

Yuka Nakamura,1,2* Rui Kano,3 Atsuhiko Hasegawa,3 and Shinichi Watanabe1

Department of Dermatology, Teikyo University School of Medicine, Tokyo,1 Departments of Veterinary Internal Medicine,2 Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa, Japan3

Received 6 November 2001/ Returned for modification 5 March 2002/ Accepted 3 April 2002

Production of interleukin-8 (IL-8) and tumor necrosis factor alpha (TNF-{alpha}) was confirmed by enzyme-linked immunosorbent assay in a medium where human epidermal keratinocytes were cocultured with Trichophyton mentagrophytes for 1 to 12 h. IL-8 and TNF-{alpha} mRNAs were also detected in the keratinocytes cocultured with T. mentagrophytes.

* Corresponding author. Mailing address: Department of Veterinary Internal Medicine, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa, Kanagawa 252-8510, Japan. Phone and fax: 81-4-6684-3647. E-mail: yuka{at}brs.nihon-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, July 2002, p. 935-937, Vol. 9, No. 4
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.4.935-937.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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