This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maurin, M.
Right arrow Articles by Raoult, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maurin, M.
Right arrow Articles by Raoult, D.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, September 2002, p. 1004-1009, Vol. 9, No. 5
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.5.1004-1009.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M against Bartonella henselae and Bartonella quintana

M. Maurin, J. M. Rolain, and D. Raoult*

Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France

Received 22 March 2002/ Returned for modification 9 May 2002/ Accepted 7 June 2002

We compared the sensitivities and specificities of indirect fluorescent antibody tests developed in our laboratory and commercially available from Focus Technologies (FT; formerly MRL Diagnostic) for detection of serum antibodies to Bartonella spp. Serum samples tested were from patients with culture- or PCR-confirmed Bartonella quintana or B. henselae infections causing cat scratch disease (CSD), chronic bacteremia, or endocarditis. At a cutoff titer of 64, the FT test had higher sensitivity than our in-house test in detecting anti-B. henselae immunoglobulin G (IgG) antibodies in CSD patients (91.2 versus 52.9%; P < 0.001). The specificity in serum samples from 85 control patients was, however, lower with the FT test (87%) than with the in-house test (98.8%) (P = 0.002). A cutoff titer of 128 improves the specificity for the FT test but lowers the sensitivity to 85%. For patients infected with B. henselae, our in-house test, but not the FT test, enabled endocarditis to be detected more reliably. With the in-house test, titers of IgG against B. henselae of >=1,024 were found only in endocarditis patients and not in CSD patients. With the FT test, 19.1% of CSD patients had titers of IgG against B. henselae of >=1,024 (P < 0.001). Our in-house technique also improved detection of anti-B. quintana antibodies in homeless patients with endocarditis. IgG titers of >=1,024 were present in 75% of serum samples, but only in 16.7% of serum samples with the FT test (P = 0.004). Since each test has advantages over the other, the serological diagnosis of Bartonella infections would benefit if both tests were used concurrently.

* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UMR 6020, IFR48, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France. Phone: (33) 4 91 38 55 17. Fax: (33) 4 91 38 77 72. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Clinical and Diagnostic Laboratory Immunology, September 2002, p. 1004-1009, Vol. 9, No. 5
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.5.1004-1009.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Raoult, D., Casalta, J. P., Richet, H., Khan, M., Bernit, E., Rovery, C., Branger, S., Gouriet, F., Imbert, G., Bothello, E., Collart, F., Habib, G. (2005). Contribution of Systematic Serological Testing in Diagnosis of Infective Endocarditis. J. Clin. Microbiol. 43: 5238-5242 [Abstract] [Full Text]  
  • Litwin, C. M, Johnson, J. M, Martins, T. B (2004). The Bartonella henselae sucB gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease. J Med Microbiol 53: 1221-1227 [Abstract] [Full Text]  
  • Tsuneoka, H., Umeda, A., Tsukahara, M., Sasaki, K. (2004). Evaluation of Indirect Fluorescence Antibody Assay for Detection of Bartonella clarridgeiae and Seroprevalence of B. clarridgeiae among Patients with Suspected Cat Scratch Disease. J. Clin. Microbiol. 42: 3346-3349 [Abstract] [Full Text]  
  • Drancourt, M., Bodaghi, B., Lepidi, H., Le Hoang, P., Raoult, D. (2004). Intraocular Detection of Bartonella henselae in a Patient with HLA-B27 Uveitis. J. Clin. Microbiol. 42: 1822-1825 [Abstract] [Full Text]  
  • Rolain, J. M., Lecam, C., Raoult, D. (2003). Simplified Serological Diagnosis of Endocarditis Due to Coxiella burnetii and Bartonella. CVI 10: 1147-1148 [Abstract] [Full Text]  
  • Levy, P. Y., Fournier, P. E., Carta, M., Raoult, D. (2003). Pericardial Effusion in a Homeless Man Due to Bartonella quintana. J. Clin. Microbiol. 41: 5291-5293 [Abstract] [Full Text]  
  • Rolain, J. M., Gouriet, F., Enea, M., Aboud, M., Raoult, D. (2003). Detection by Immunofluorescence Assay of Bartonella henselae in Lymph Nodes from Patients with Cat Scratch Disease. CVI 10: 686-691 [Abstract] [Full Text]  
  • TEA, A., ALEXIOU-DANIEL, S., ARVANITIDOU, M., DIZA, E., ANTONIADIS, A. (2003). OCCURRENCE OF BARTONELLA HENSELAE AND BARTONELLA QUINTANA IN A HEALTHY GREEK POPULATION. Am J Trop Med Hyg 68: 554-556 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»