This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collins, M. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collins, M. T.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1367-1371, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1367-1371.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Interpretation of a Commercial Bovine Paratuberculosis Enzyme-Linked Immunosorbent Assay by Using Likelihood Ratios

M. T. Collins*

School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 53706

Received 9 April 2002/ Returned for modification 16 August 2002/ Accepted 4 September 2002

Evidence-based medicine encourages the use of quantitative diagnostic test results to estimate the probability of a particular diagnosis. Likelihood ratios (LRs) are among the best tools for maximizing the diagnostic information gained from diagnostic assays that provide results on a continuous scale. They provide the odds that an animal with a particular test result actually has the disease in question based on the magnitude of the test result. A commercial enzyme-linked immunosorbent assay (ELISA) was used to test sera from 143 dairy cattle infected with Mycobacterium paratuberculosis and 2,974 cattle free of this infection. This assay transforms ELISA reader optical density values into sample-to-positive (S/P) ratios. The LR was calculated for S/P results from 0.00 to 1.00 at 0.05-S/P unit intervals. LRs were directly but not linearly correlated with ELISA S/P ratios (r2 = 0.94). The mathematical function describing the relationship between the ELISA S/P ratio and the LR was LR = 265 x (S/P value)2.03. LRs were also directly related to the frequency of animals testing positive for paratuberculosis by fecal culture and other serologic tests. Based on these LRs, guidelines for interpretation and application of this ELISA for the diagnosis and control of paratuberculosis in dairy cattle herds are recommended.

* Mailing address: School of Veterinary Medicine, University of Wisconsin—Madison, 2015 Linden Dr., Madison, WI 53706-1102. Phone: (608) 262-8457. Fax: (608) 265-6463. E-mail: mcollin5{at}facstaff.wisc.edu.

Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1367-1371, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1367-1371.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Elzo, M. A., Rae, D. O., Lanhart, S. E., Wasdin, J. G., Dixon, W. P., Jones, J. L. (2006). Factors associated with ELISA scores for paratuberculosis in an Angus-Brahman multibreed herd of beef cattle. J ANIM SCI 84: 41-48 [Abstract] [Full Text]  
  • Toft, N., Nielsen, S. S., Jorgensen, E. (2005). Continuous-Data Diagnostic Tests for Paratuberculosis as a Multistage Disease. J DAIRY SCI 88: 3923-3931 [Abstract] [Full Text]  
  • Collins, M. T., Wells, S. J., Petrini, K. R., Collins, J. E., Schultz, R. D., Whitlock, R. H. (2005). Evaluation of Five Antibody Detection Tests for Diagnosis of Bovine Paratuberculosis. CVI 12: 685-692 [Abstract] [Full Text]  
  • Bernstein, C. N., Blanchard, J. F., Rawsthorne, P., Collins, M. T. (2004). Population-Based Case Control Study of Seroprevalence of Mycobacterium paratuberculosis in Patients with Crohn's Disease and Ulcerative Colitis. J. Clin. Microbiol. 42: 1129-1135 [Abstract] [Full Text]  
  • Naugle, A. L., Shulaw, W. P., Saville, W. J. A., Wittum, T. E., Byrum, B., Collins, M. T. (2003). Calculation Method for Likelihood Ratios Dictates Interpretation. CVI 10: 729-730 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»