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Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1379-1381, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1379-1381.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of a Single Monoclonal Antibody To Determine the Susceptibilities of Herpes Simplex Virus Type 1 and Type 2 Clinical Isolates to Acyclovir

Christine Chutkowski,1 Betty Olson,1 Ann McDonough,1 James Mahoney,2 and James J. McSharry1*

Center for Immunology and Microbial Disease, Albany Medical Center, Albany, New York 12208,1 Regional Virology Laboratory, St. Joseph's Hospital, McMaster University, Hamilton, Ontario, Canada2

Received 17 June 2002/ Returned for modification 30 July 2002/ Accepted 13 August 2002

This report describes a flow cytometry drug susceptibility assay that uses a single fluorochrome-labeled monoclonal antibody to determine the acyclovir susceptibilities of herpes simplex virus (HSV) type 1 or type 2 clinical isolates. This assay yields 50% effective doses (drug concentrations that reduce the number of antigen-positive cells by 50%) for HSV clinical isolates that are equivalent to those obtained with the plaque reduction assay.

* Corresponding author. Mailing address: Center for Immunology and Microbial Disease, Mail Code 151, Albany Medical College, 47 New Scotland Ave., Albany, NY 12208. Phone: (518) 262-5174. Fax: (518) 262-5748. E-mail: mchsarj{at}mail.amc.edu.

Clinical and Diagnostic Laboratory Immunology, November 2002, p. 1379-1381, Vol. 9, No. 6
1071-412X/02/$04.00+0     DOI: 10.1128/CDLI.9.6.1379-1381.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McSharry, J. J., McDonough, A. C., Olson, B. A., Drusano, G. L. (2004). Phenotypic Drug Susceptibility Assay for Influenza Virus Neuraminidase Inhibitors. CVI 11: 21-28 [Abstract] [Full Text]  


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