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Clin. Vaccine Immunol. doi:10.1128/CVI.00012-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Prevention of assay interference in the LIAISON® infectious serology tests

Algemeen Medisch Laboratorium, Immunology Department, Desguinlei 88, 2018 Antwerpen, Belgium

^* To whom correspondence should be addressed. Email: mario.berth{at}aml-lab.be.

	Abstract

Immunoassay interference causing unexpected reactive results in magnetic microparticle based assays was detected. A systematic evaluation of LIAISON® EBV IgM showed that 5% of the positive results (0.4% of tested samples) could be explained by such interference. Adding chemical blocking reagents (polyvinylpyrrolidone and polyvinyl alcohol) to the assay buffers partially prevented this phenomenon.