CVI Accepts, published online ahead of print on 25 April 2007

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Clin. Vaccine Immunol. doi:10.1128/CVI.00104-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Milk and Serum J5-Specific Antibody Responses, Milk Production Change and Clinical Effects Following Intramammary Escherichia coli Challenge For J5 Vaccinate and Control Cows

David J. Wilson^*, Bonnie A. Mallard, Jeanne L. Burton, Ynte H. Schukken, and Yrjo T. Grohn

Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14850; Department of Pathobiology, University of Guelph, Guelph, Ontario, CA; Department of Animal Science, Michigan State University, East Lansing, MI 48824

^* To whom correspondence should be addressed. Email: David.Wilson{at}usu.edu.

Holstein dairy cows (4 J5 vaccinates, 4 controls) selected for no recorded intramammary disease and low somatic cell count (SCC) during the previous lactation were challenged by intramammary infusion of Escherichia coli. Vaccination with J5 was at -8 wk and again -4 wk from anticipated calving date. Cows were challenged at 8 - 16 days in milk (DIM). Shedding of E. coli in milk was significantly higher among controls than vaccinates (no shedding) from 6 h to 21 h post-challenge. From 21 h to 132 h post-challenge, SCC in challenged quarters of controls (5,429,000/ml) was significantly higher than that of vaccinates (490,000/ml). On the day after challenge, control cows lost 8 kg of milk production, while vaccinates gained 0.5 kg, a significant difference. In serum immediately prior to challenge, J5-specific IgG1 was significantly higher, IgG2 was nearly significantly higher, and IgM was the same in J5 vaccinates relative to controls. Vaccinates had proportionally more IgG2 in serum post-calving and in the first 12 h following challenge, and less IgG2 in milk 24 h after challenge than controls, approaching statistical significance. The ratio of J5-specific IgG1 and IgG2 combined compared to IgM was significantly higher in vaccinates than controls in pre-challenge serum (15.8, 3.2 ratios respectively) and milk (5.0, 1.3). Cows with higher IgM titers in milk 12 h post-challenge lost significantly more milk. Vaccination with J5 was significantly associated with higher production of J5-specific IgG1 and IgG2 in early lactation, reduced SCC, faster clearance of E. coli from milk, and less milk production loss following intramammary challenge.

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